Composite
Part:BBa_K5477026:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
pRET2-NCOA - receptor module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal NheI site found at 182
Illegal NheI site found at 3547
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal BamHI site found at 3049 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1785
Illegal EcoRI site found at 2407
Illegal SpeI site found at 1234
Illegal SpeI site found at 1535
Illegal SpeI site found at 2602
Illegal PstI site found at 1294
Illegal AgeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This module supports the AhR-mediated response to toxic ligands like PAHs, dioxins and PCBs by binding to the XRE() in our reporter module facilitating the transcription of NanoLuc (). The composite part was cloned using the method of USER-cloning into YCp-W. YCp-W is a centromeric plasmid used in yeast that carries a TRP1 marker, allowing for selection in tryptophan auxotrophic yeast strains. Like other CEN plasmids, YCp-W contains a CEN sequence, ensuring that it replicates and segregates similarly to yeast chromosomes. This low-copy plasmid (typically maintained at one to two copies per cell) provides stable maintenance within yeast cells.
Source
Homo sapiens and Saccharomyces cerevisiae